Expression of Rock2 and TGF-β1 mRNA and glucocorticoid intervention in asthmatic rats

• Published in: Zhongguo dang dai er ke za zhi Vol. 12; no. 11; p. 877
• Main Authors: Zheng, Chang-Hua, Tong, Xia-Sheng, Li, Bin-Fei, Zhang, Wei-Xi, Li, Chang-Chong
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2010
• Subjects: Animals; Asthma - drug therapy; Asthma - metabolism; Budesonide - therapeutic use; Dexamethasone - therapeutic use; Glucocorticoids - therapeutic use; Lung - metabolism; Lung - pathology; Male; Rats; Rats, Sprague-Dawley; rho-Associated Kinases - genetics; RNA, Messenger - analysis; Transforming Growth Factor beta1 - genetics
• ISSN: 1008-8830

To study the roles of rhoassociated coiled coil forming protein kinase 2 (Rock2) and transforming growth factor-β1 (TGF-β1) mRNA in acute asthma and the effect of glucocorticoid intervention on the Rock2 and TGF-β1 mRNA expression in rats. Forty-eight male rats were randomly divided into 4 groups (n=12 each): asthma, control, dexamethasone treated (DXM) and budesonide treated (BUD). Rat model of asthma was prepared by the ovalbumin (OVA) challenge. The animals were sacrificed 24 hrs after the last challenge. The total cell number and differentiation cell number were counted in bronchoalveolar lavage fluid (BALF). The protein expression of Rock2 was ascertained by immunohistochemistry and the mRNA expression of TGF-β1 was ascertained by hybridization in situ. The pathological changes in the BUD and the DXM groups were alleviated when compared with the asthma group. The total cell number and the percentage of eosinophil (EOS), polymorphonuclear leukocytes (PMN) and lymphocytes (Lym) in BALF in the asthma group