Optimization of cultivation conditions of alpha-L-rhamnosidases producers-- representatives of different taxonomic groups of microorganisms

• Published in: Mikrobiolohichnyĭ zhurnal (Kiev, Ukraine : 1993) Vol. 73; no. 3; p. 46
• Main Authors: Hudzenko, E V, Borzova, N V, Varbanets', L D
• Format: Journal Article
• Language: Ukrainian
• Published: Ukraine 01.05.2011
• Subjects: Bacillus - classification; Bacillus - enzymology; Bacillus - growth & development; Bacteriological Techniques - methods; Cryptococcus - classification; Cryptococcus - enzymology; Cryptococcus - growth & development; Eupenicillium - classification; Eupenicillium - enzymology; Eupenicillium - growth & development; Glycoside Hydrolases - biosynthesis; Mycology - methods; Temperature
• ISSN: 1028-0987

Influence ofsome technological parameters of cultivation ofproducers Cryptococcus albidus, Eupenicillinum erubescens, Bacillus sp. on the process of synthesis of extracellular enzyme alpha-L-rhamnosidase has been studied. The authors have determined optimal sources of carbon (0.2-0.3% rhamnose) and nitrogen (0.2% sodium nitrate for C. albidus and E. erubescens and ammonium sulphate for Bacillus sp.) (the ratio 1:2), cultivation temperature (28 degrees C, 25 degrees C, 42 degrees C, respectively) for maximum synthesis of alpha-L-rhamnosidase. Use of the medium with initial pH value from 4 to 8 was most efficient for all the studied strains. The maximum level of alpha-L-rhamnosidase activity of E. erubescens and Bacillus sp. was established at the value of sulphite number of 0.44, while for C. albidus--it was 0.56. Maximum alpha-L-rhamnosidase activity of C. albidus, E. erubescens, Bacillus sp. is achieved at 4, 8 days and 27 hours of cultivation, respectively. The cultures being grown in selected conditions, the alpha-L-rhamnosidase synthesis has increased by 30, 50 and 20%, respectively.