Inhibitory effect and acting mechanism of Tongxinluo on IL-1beta-mediated coronary intimal hyperplasia and 5-hydroxytryptamine-induced coronary vasospasm in small swine

• Published in: Zhongguo Zhong xi yi jie he za zhi Vol. 28; no. 7; p. 627
• Main Authors: Guan, Qi-Gang, Zeng, Ding-Yin, Sun, Xi-Zhuo
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2008
• Subjects: Animals; Coronary Vasospasm - chemically induced; Coronary Vasospasm - drug therapy; Coronary Vasospasm - genetics; Coronary Vasospasm - metabolism; Disease Models, Animal; Drugs, Chinese Herbal - therapeutic use; Gene Expression - drug effects; Humans; Interleukin-1beta - genetics; Interleukin-1beta - metabolism; Male; Random Allocation; rho-Associated Kinases - genetics; rho-Associated Kinases - metabolism; Serotonin - adverse effects; Swine
• ISSN: 1003-5370

To observe the inhibitory effect of Tongxinluo (TXL) on coronary vaso spasm in small swine in vivo, and to investigate its possible acting mechanism. The model of coronary atherosclerosis in 16 male small swines was established by left thoracotomy after anesthesia, isolated the sections of left anterio-descending branch and proximal end of rotator branch with similar outer diameter, and encapsulated them with paper-towel holding 2.5 microg interleukin-1beta. Two weeks later, the condition of coronary vasospasm induced by catheter intra-coronary injection of 5-hydroxytryptamine (5-HT, 10 microg/kg) was observed through coronary artery contrast examination. The 12 swines with successfully formed coronary vaso spasm were randomly divided into 2 groups, the TXL group and the control group. They were fed with special diet, but TXL 1 g/(kg d) was administered additionally to the TXL group for 4 weeks. The observation on coronary vasospasm was repeated 1 week after discontinuation of TXL treatment, then the animals