Investigation on induced expansion of erythroid cells from cord blood CD34(+) cells in vitro

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 17; no. 3; p. 787
• Main Authors: Jia, Yan-Jun, Liu, Jiang, Zhang, Ke-Ying, Shan, Xiao-Yan, Li, Wei, He, Xiao-Mei, Wang, Li-Jun, Liu, Na, Wang, Lin, Cui, Shuang, Ni, Lei, Zhao, Bo-Tao, Gong, Zhi-Yin, Wang, Dong-Mei, Gao, Song-Ming, Zhang, Zhi-Xin
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2009
• Subjects: Antigens, CD34; Cell Culture Techniques - methods; Cell Differentiation; Fetal Blood - cytology; Hematopoietic Stem Cells - cytology; Humans
• ISSN: 1009-2137

This study was aimed to investigate a beneficial approach for resolving the deficiency of blood source, preventing the infection resulting from blood transfusion and overcoming the knotty match of patients with rare blood group by using massive expansion of erythroid cells from cord blood CD34(+) cells in vitro. The CD34(+) cells from human cord blood were cultured in serum-free medium supplemented with stem cell factor (SCF), interleukin-3 (IL-3) and erythropoietin (EPO) for 1 week, then expansion and differentiation of CD34(+) cells into erythroid cells were supported by co-culture with human mesenchymal stem cells (MSCs) derived from bone marrow for 2 weeks. The results indicated that after culture for 23 days, the expansion multiple of total cell number reached 2.52 x 10(5), and over 95% of these cells were erythroid cells as compared with less than 1% of myelomonocytic (CD14(+) or CD15(+)) cells and megakaryocytic (CD41(+)) cells. However, the culture system without MSC support was significantly disadvan