Effects of minimal persistent inflammation on nasal mucosa of experimental allergic rhinitis in guinea pigs

• Published in: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery Vol. 43; no. 7; p. 499
• Main Authors: Lei, Fei, Cui, Ya-Nan, Jiang, Yun-Peng, Kong, Hong, Zhu, Dong-Dong, Dong, Zhen
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2008
• Subjects: Animals; Disease Models, Animal; Guinea Pigs; Inflammation; Male; Matrix Metalloproteinase 9 - metabolism; Nasal Mucosa - metabolism; Rhinitis, Allergic, Seasonal - metabolism; Transforming Growth Factor beta1 - metabolism
• ISSN: 1673-0860

To establish an animal model of minimal persistent inflammation (MPI) of allergic rhinitis in guinea pigs and to investigate the changes of nasal mucosa. The expression of transforming growth factor-beta1 (TGF-beta1) and matrix metalloproteinases-9 (MMP-9) were discussed. Thirty male Hartley guinea pigs were randomly divided into two groups: MPI model group and control group randomly, with fifteen animals in each group. Guinea pigs from MPI model group were sensitized intraperitoneally by injection of suspension of ovalbumin (OVA) and aluminum hydroxide in 0.9% physiological saline. Then, repeated local booster sensitization with low concentration of OVA suspension into the nasal cavity was performed to establish MPI models. Alcian blue-periodic acid-Schiff (AB-PAS) staining and Masson's trichrome (MT) staining were used to determine the number of goblet cells and collagen deposition within the basement membrane of epithelium. The expression and distribution of TGF-beta1 and MMP-9 in nasal mucosa were estimat