Effect of microwave radiation on primary cultured Sertoli cells

• Published in: Zhonghua laodong weisheng zhiyebing zazhi Vol. 27; no. 9; p. 530
• Main Authors: Gao, Xiao-fang, Wang, Shui-ming, Peng, Rui-yun, Wang, Li-feng, Zuo, Hong-yan, Gao, Ya-bing, Dong, Qi, Dong, Bo
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2009
• Subjects: Animals; Apoptosis - radiation effects; Calcium - metabolism; Cell Cycle - radiation effects; Cells, Cultured; Male; Microwaves - adverse effects; Rats; Rats, Wistar; Sertoli Cells - metabolism; Sertoli Cells - pathology; Sertoli Cells - radiation effects
• ISSN: 1001-9391

To explore whether microwave radiation may cause injury of primary cultured Sertoli cells. The model of primary cultured Sertoli cells in vitro was established, which was radiated by microwave with average power density 0, 30 and 100 mW/cm(2) for five minutes. The changes of cell cycle, apoptosis and death, and intracellular Ca2+ concentration in the Sertoli cells were measured at sixth hours through Annexin V-PI double labeling and Fluo-3-AM labeling, flow cytometry combined with laser scanning confocal microscopy after microwave exposure. The numbers of Sertoli cells were obviously reduced in G0-G1 and G2-M phase (62.57% +/- 3.22% and 8.25% +/- 1.75%) and increased in S phase (29.17% +/- 4.87%) compared with the control groups (79.18% +/- 0.24%, 11.17% +/- 0.50% and 9.64% +/- 0.62%) (P < 0.05 or P < 0.01), but the changes of rate of apoptosis and death and intracellular Ca2+ concentration showed no difference at 6 h after exposure to 30 mW/cm(2) microwave. There was a significant increase in the Sertoli cel