Study on molecular typing by pulsed-field gel electrophoresis of food Listeria monocytogenes isolates from eight province (municipalities) of China

To analyse the molecular types of Listeria (L.) monocytogenes, and to construct the standard China L. monocytogenes pulsed-field gel electrophoresis (PFGE) subtyping database, using the international standardized L. monocytogenes-PFGE protocol. 118 L. monocytogenes strains isolated from 8 provinces...

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Published inZhōnghuá liúxíngbìng zázhì Vol. 28; no. 5; p. 464
Main Authors Zhu, Ren-fa, Gong, Zhao-long, Ye, Chang-yun, Cui, Zhi-gang, Jin, Dong, Ran, Lu, Xu, Ke, Wang, Lian-xiu, Chen, Wei-wei
Format Journal Article
LanguageChinese
Published China 01.05.2007
Subjects
China
Electrophoresis, Gel, Pulsed-Field
Food Microbiology
Listeria monocytogenes - classification
Listeria monocytogenes - genetics
Listeria monocytogenes - isolation & purification
Phylogeny
China
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Summary:To analyse the molecular types of Listeria (L.) monocytogenes, and to construct the standard China L. monocytogenes pulsed-field gel electrophoresis (PFGE) subtyping database, using the international standardized L. monocytogenes-PFGE protocol. 118 L. monocytogenes strains isolated from 8 provinces or municipalities of China were subtyped according to L. monocytogenes-PFGE protocol. 118 strains of L. monocytogenes were divided into 39 subtypes. In the 39 subtypes, 37 strains (31.36%) were GX6A160004 pattern, showing it was the predominant Lm subtype in China. Data from molecular typing suggested that the predominant Lm strains were distributed in different regions of China. PulseNet China-Lm database was constructed which was valuable for the molecular subtyping-based surveillance of L. monocytogenes.
ISSN:0254-6450