16S rRNA gene-based molecular methods to monitor clostridium cluster IV community in the colon of piglets

To investigate the change of Clostridium cluster IV community in the colon of piglets from 7 to 35 days of age, and its correlation with butyrate concentration. Three litters of neonatal piglets were used. One piglet from each litter was sacrificed randomly at the age of 7, 14, 21 (weaning day), 24...

Full description

Saved in:
Bibliographic Details
Published inWei sheng wu hsüeh pao Vol. 50; no. 10; p. 1373
Main Authors Bian, Gaorui, Xie, Fei, Su, Yong, Zhu, Weiyun
Format Journal Article
LanguageChinese
Published China 01.10.2010
Subjects
Animals
Clostridium - genetics
Clostridium - physiology
Cluster Analysis
Colon - microbiology
Denaturing Gradient Gel Electrophoresis - methods
Genes, rRNA - genetics
Phylogeny
Polymerase Chain Reaction - methods
RNA, Ribosomal, 16S - analysis
RNA, Ribosomal, 16S - genetics
Swine
Online AccessGet more information

Cover

More Information
Summary:To investigate the change of Clostridium cluster IV community in the colon of piglets from 7 to 35 days of age, and its correlation with butyrate concentration. Three litters of neonatal piglets were used. One piglet from each litter was sacrificed randomly at the age of 7, 14, 21 (weaning day), 24 and 35 days, digestive samples in the colon were collected. The concentration of volatile fatty acid (VFA) was determined by gas chromatography. 16S rRNA gene-based denaturing gradient gel electrophoresis (DGGE) and real-time PCR were used for qualitative and quantitative analysis of Clostridium cluster IV community. Similarity analysis of DGGE profile revealed that samples from piglets at the age of 7 days formed a coherent cluster with indices above 90%, no significant changes in Clostridium cluster IV community were found around weaning period. Real-time PCR analysis showed that 16S rRNA gene copies of total bacteria in the colon of piglets decreased significantly 3 days after weaning, this tendency was in accor
ISSN:0001-6209