Study on the enrichment and purification of total flavonoids in Microcos paniculata by macroporous adsorption resin

• Published in: Zhong yao cai Vol. 32; no. 4; p. 601
• Main Authors: Li, Kun-Ping, Pan, Tian-Ling, Gao, Chong-Kai, Li, Wei-Min
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2009
• Subjects: Adsorption; Chromatography, High Pressure Liquid - methods; Ethanol - chemistry; Flavonoids - isolation & purification; Hydrogen-Ion Concentration; Plant Leaves - chemistry; Plants, Medicinal - chemistry; Resins, Synthetic - chemistry; Spectrophotometry, Ultraviolet; Technology, Pharmaceutical - methods; Tiliaceae - chemistry
• ISSN: 1001-4454

To establish an optimum enrichment and purification process of total flavonoids in Microcos paniculata by macroporous resins. Five kinds of resins were compared and the best one was chosen. Then the parameters of the process were optimized by single factor tests, uniform design and statistical methods. DI01 was selected for its excellent adsorption and desorption properties, 70% ethanol was found to be the best elution solution. As far as the yield was considered, the best result was based on the followings: feed rate-1.0 BV/h, elution flow rate-2.0 BV/h, sample concentration-7.88 mg/mL, eluting agent amount-2.0 BV, pH value 4.8; then the yield reached 90.18% and the purity was 54.37%. If the purity was considered, the best parameters wereas follows: feed rate-1.0 BV/h, elution flow rate-2.0 BV/h, sample concentration-2.0 mg/mL, eluting agent amount-2.8 BV, pH value 7.8; then the purity reached 61.77% and the yield was 80.25%. The total flavonoids of Microcos paniculata can be effectively purificated and sepa