Mechanism of Smad 3 signaling pathway and connective tissue growth factor in the inhibition of form deprivation myopia by pirenzepine

• Published in: Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban Vol. 34; no. 4; p. 349
• Main Authors: Ji, Xueying, Zhang, Jinsong, Wang, Yanting, Sun, Hongliang, Jia, Peisheng
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2009
• Subjects: Animals; Connective Tissue Growth Factor - metabolism; Guinea Pigs; Humans; Muscarinic Antagonists - administration & dosage; Myopia - prevention & control; Pirenzepine - administration & dosage; Random Allocation; Sensory Deprivation; Signal Transduction - drug effects; Smad3 Protein - metabolism
• ISSN: 1672-7347

To observe the inhibitive effect of pirenzepine on form deprivation myopia in guinea pigs and to explore the mechanism of Smad3 signaling pathway and connective tissue growth factor (CTGF) in the inhibition of myopia by pirenzepine. Forty 1-week-old guinea pigs of either sex were randomly divided into 4 groups: a control group (Group I), a form deprivation group (Group II), a pirenzepine ophthalmic solution group (Group III), and a sodium chloride ophthalmic solution group (Group IV). Translucent blinders were used in the right eyes of Group II, III and IV. The left eyes were not given any treatment as the normal control group. Covered eyes of Group III and IV were given 3% pirenzepine ophthalmic solution and 0.1% azone ophthalmic solution respectively twice every day. Six weeks later, refraction and axial length were measured at the end of the experiment, and immunohistochemistry and Western blot were used to analyze the expression levels of Smad3 and CTGF in the sclera of all 4 groups. There was no signific