Clinical application of telomerase RNA component gene amplification assay in cervical lesions.

To investigate the significance of genomic amplification of the telomerase RNA component (TERC) gene to serve as a genetic biomarker in the screening of cervical lesions. A total of 715 cases were recruited, with liquid-based cytology diagnosis as normal (n = 347), atypical squamous cells of undeter...

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Published inChung-hua fu chʿan kʿo tsa chih Vol. 44; no. 12; p. 883
Main Authors Jiang, Jing, Wei, Li-Hui, Wu, Rui-Fang, Zhang, Guo, Wulan, Na, Li, Jing-Ran, Li, Yi-Bing, Tu, Zheng, Zhou, Yan-Qiu, Zhao, Yun, Chen, Zhong
Format Journal Article
LanguageChinese
Published China 01.12.2009
Subjects
Cervical Intraepithelial Neoplasia
Gene Amplification
Humans
In Situ Hybridization, Fluorescence
Uterine Cervical Dysplasia
Uterine Cervical Neoplasms
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Summary:To investigate the significance of genomic amplification of the telomerase RNA component (TERC) gene to serve as a genetic biomarker in the screening of cervical lesions. A total of 715 cases were recruited, with liquid-based cytology diagnosis as normal (n = 347), atypical squamous cells of undetermined significance (ASCUS, n = 180), atypical squamous cells cannot exclude a high-grade lesion (ASC-H, n = 13), low-grade squamous intraepithelial lesions (LSIL, n = 115), high-grade squamous intraepithelial lesions (HSIL, n = 59) and atypical glandular cells (AGC, n = 1). The remaining cervical cells in the cytological preserving fluid were analyzed using a two-color fluorescence in situ hybridization (FISH) probe targeted to chromosome 3q26 containing TERC gene. The TERC gene findings were compared to the cytological and histological detected results, as well as high-risk human papillomavirus (HPV) detected results. Genomic amplification of TERC gene was found in 5.8% of normal specimens, 22.2% of ASCUS, 30.8% o
ISSN:0529-567X