Study on reversion of malignant phenotype of glioma by siRNA targeting p75 neurotrophin receptor

• Published in: Zhonghua bing li xue za zhi Vol. 39; no. 6; p. 400
• Main Authors: Zhao, Zhan-kao, Jiang, Zhong-min, Liu, Xiao-zhi, Chen, Yan-ting, Jia, Wen-juan
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2010
• Subjects: Animals; Apoptosis; Brain Neoplasms - genetics; Brain Neoplasms - metabolism; Brain Neoplasms - pathology; Cell Line, Tumor; Cell Movement; Cell Proliferation; Cyclin D2 - metabolism; Gene Silencing; Glioma - genetics; Glioma - metabolism; Glioma - pathology; Humans; Male; Mice; Mice, Nude; Neoplasm Invasiveness; Neoplasm Transplantation; Nerve Growth Factor - metabolism; Random Allocation; Receptor, Nerve Growth Factor - genetics; Receptor, Nerve Growth Factor - metabolism; RNA, Messenger - metabolism; RNA, Small Interfering - genetics
• ISSN: 0529-5807
• DOI: 10.3760/cma.j.issn.0529-5807.2010.06.009

To study the therapeutic efficacy of siRNA fragments silencing p75 neurotrophin receptor (p75(NTR)), which may be a key regulator of glioma cell apoptosis and invasion. The siRNA sequence fragments targeting p75(NTR) were designed and transferred into human glioma cell line U251. RT-PCR and immunocytochemistry method were used to explore the expression of p75(NTR) mRNA and protein. Cell adhesion assay was employed to detect cellular adhesion ability, and soft agar clone formation assay was adopted to identify oncogenicity, and a U251 glioma model was established in nude mice. The intracranial tumor volume was detected by MRI. The expression of p75(NTR), NGF and cyclin D2 were identified using immunohistochemistry. Cell apoptosis was detected by apoptosis kit in situ. The siRNA fragments targeting p75(NTR) were capable of decreasing mRNA and protein expression of p75(NTR) in U251 glioma cell line. Both the cellular adhesion ability and oncogenicity were weakly relevant. The p75(NTR) expression level was negati