A mouse model of myelofibrosis induced by high dose of recombinant human erythropoietin

Tn order to set up a mouse model of myelofibrosis (MF) induced with high dose recombinant human erythropoietin (rhEPO). 60 mice were collected and divided into EPO and control groups, the former was injected with rhEPO and the latter with normal saline intraperitoneally. 5 mice from each group were...

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Published inZhongguo shi yan xue ye xue za zhi Vol. 19; no. 4; p. 1043
Main Authors Zhang, Hua-Mei, Gao, Jing-Tao, Zhao, Shi-Xuan, Meng, Zhao-Ting, Wang, Hui-Jun, Li, Xiao-Mei, Zhu, Xiao-Fan, Ru, Yong-Xin
Format Journal Article
LanguageChinese
Published China 01.08.2011
Subjects
Animals
Disease Models, Animal
Erythropoietin - adverse effects
Female
Humans
Mice
Mice, Inbred Strains
Primary Myelofibrosis - chemically induced
Recombinant Proteins - adverse effects
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Summary:Tn order to set up a mouse model of myelofibrosis (MF) induced with high dose recombinant human erythropoietin (rhEPO). 60 mice were collected and divided into EPO and control groups, the former was injected with rhEPO and the latter with normal saline intraperitoneally. 5 mice from each group were executed on day 6, 30, 60, 90, 120 and 150 respectively. Their WBC count, Hb level, MCV, RDW and platelet amount were measured by automatic blood cell analyzer; CD34(+) cell ratio in bone marrow were analyzed by flow cytometry; liver and spleen coefficients were measured; pathological changes of liver, spleen, femur were observed by HE staining and reticular fibers staining; cortex thickness, femoral canal diameter and lumbar spine density were determined by computerized tomography (CT). The results indicated that as compared with normal control group in EPO induced group, WBC count was increased slightly in whole period, but without statistic significance (p > 0.05), Hb level and RDW increased at day 6 and 30 sign
ISSN:1009-2137