Detection of antimalarial activity for new compounds by isotopic microtest

Two clone lines (Dd2 and 3D7) of Plasmodium falciparum were cultivated continuously in human erythrocytes at 37 degrees C in RPMI 1640 medium with human serum and subjected to 6% sorbitol treatment 2 times in order to obtain highly synchronized cultures. The second generation parasites after the tre...

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Published inZhongguo jishengchongxue yu jishengchongping zazhi Vol. 27; no. 1; p. 83
Main Authors Chen, Zhao-guo, Moreno, Alicia, Benito, Agustin, Moreno, Marta, Berzosa, Pedro J, de Lucio, Aida, Moyano, Eva
Format Journal Article
LanguageChinese
Published China 28.02.2009
Subjects
Antimalarials - analysis
Antimalarials - pharmacology
Drug Evaluation, Preclinical
Erythrocytes - parasitology
Humans
Parasitic Sensitivity Tests
Plasmodium falciparum - drug effects
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Summary:Two clone lines (Dd2 and 3D7) of Plasmodium falciparum were cultivated continuously in human erythrocytes at 37 degrees C in RPMI 1640 medium with human serum and subjected to 6% sorbitol treatment 2 times in order to obtain highly synchronized cultures. The second generation parasites after the treatment were diluted with human RBC to be a suspension of P. falciparum-human RBC at 2.5% hematocrit and 0.5% parasitemia, and 2 microCi/ml of 8-3H-hypoxanthine was added. Isotopic microtest was employed to detect the antimalarial activity for 20 new compounds. Results revealed that the 20 compounds showed no anti-malarial activity, while the control drugs, chloroquine and quinine, exhibited high efficacy, indicating that the isotopic microtest is a stable and reproducible assay for screening new antimalarials.
ISSN:1000-7423