Identification of a novel HLA allele DRB11462 by sequence-based typing

This study was to identify a novel HLA-DRB1 allele in Chinese population by nucleotide sequence ana- lysis. The HLA typing of genes was performed by PCR-SSO and PCR-SSP, the ambiguous novel allele was identified by DNA sequence analysis. The results showed that the sequence of this new allele differ...

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Bibliographic Details
Published inZhongguo shi yan xue ye xue za zhi Vol. 17; no. 2; p. 493
Main Authors Cui, Shuang, Shan, Xiao-Yan, He, Xiao-Mei, Liu, Na, Wang, Lin, Li, Wei, Wang, Li-Jun, Ni, Lei, Wang, Dong-Mei, Zhao, Bo-Tao, Gong, Zhi-Yin, Zhang, Zhi-Xin
Format Journal Article
LanguageChinese
Published China 01.04.2009
Subjects
Alleles
Asian Continental Ancestry Group - genetics
Histocompatibility Testing
HLA-DR Antigens - genetics
HLA-DRB1 Chains
Humans
Molecular Sequence Data
Sequence Analysis, DNA
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Summary:This study was to identify a novel HLA-DRB1 allele in Chinese population by nucleotide sequence ana- lysis. The HLA typing of genes was performed by PCR-SSO and PCR-SSP, the ambiguous novel allele was identified by DNA sequence analysis. The results showed that the sequence of this new allele differed from DRB1*140101 by one nucleotide substitution at position 256 in exon 2 (G- > A), resulting in an amino acid change from Ala to Thr at codon 57. In conclusion, this allele is a novel one, which has been officially given the name DRB1*1462 by the WHO nomenclature committee in January 2006.
ISSN:1009-2137