E1A gene transfection of human undifferentiated thyroid cancer cell line HTC/3 by nanoparticles

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 29; no. 12; p. 884
• Main Authors: He, Xiang-Liang, He, Dong-Hua, Liao, Xiao-Xing, Zhan, Hong, Ma, Zhong-Fu, Wang, Xi-Fu, Li, Qing, Li, Xin, Li, Yu-Jie
• Format: Journal Article
• Language: Chinese
• Published: China 01.12.2007
• Subjects: Adenovirus E1A Proteins - biosynthesis; Adenovirus E1A Proteins - genetics; Adenovirus E1A Proteins - physiology; Apoptosis - radiation effects; Cell Cycle - radiation effects; Cell Line, Tumor; Cell Proliferation; DNA - genetics; Humans; Lactic Acid - chemistry; Nanoparticles; Particle Size; Plasmids; Polyglycolic Acid - chemistry; RNA, Messenger - metabolism; Thyroid Neoplasms - metabolism; Thyroid Neoplasms - pathology; Transfection; X-Rays
• ISSN: 0253-3766

To prepare nanoparticles containing E1A gene and observe the efficiency and feasibility of transfecting E1A gene into human undifferentiated thyroid cancer cell line HTC/3. To examine the sensitivity of transgene cells to X-ray and X-ray-induced apoptosis in those cells. Nanoparticle-DNA complex was prepared with PLGA coating adenoviral early expression gene E1A, and the package efficiency, release progress in vitro, and size of the complex were determined. The nanoparticle-DNA was transfected into the HTC/3 cells. Lipofectamine was used to transfect E1A gene as a control. RT-PCR was used to examine E1A gene mRNA expression in the transfected cells. The survival ratio of HTC/3-E1A and control cells, and the growth inhibition ratio induced by different doses of X-ray in HTC/3-E1A cells were examined by MTT assay. The apoptosis in HTC/3-E1A cells induced by 2 Gy X-ray iradiation was examined by flow cytometry and DNA electrophoresis. The package efficiency, release progress in vitro, and size of the nanoparticl