Clinical and molecular cytogenetic studies of a case of B-lineage acute lymphoblastic leukemia with t(14;14)(q11;q32)

• Published in: Zhonghua yi xue yi chuan xue za zhi Vol. 29; no. 2; p. 137
• Main Authors: Han, Yong-sheng, Xue, Yong-quan, Zhang, Jun
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2012
• Subjects: Adult; Chromosomes, Human, Pair 14; Cytogenetics - methods; Female; Follow-Up Studies; Genetic Predisposition to Disease; Humans; Karyotype; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - genetics; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma - pathology
• ISSN: 1003-9406
• DOI: 10.3760/cma.j.issn.1003-9406.2012.02.004

To report on a rare case of B-lineage acute lymphoblastic leukemia (B-ALL) with t(14;14) (q11;q32) and clarify its clinical and molecular cytogenetic features. Clinical data of a B-ALL patient with t(14;14) (q11;q32) were analyzed. After 24 hour of unstimulated culturing, chromosome specimens of bone marrow cells were prepared with regular method, and R-banding was used for karyotype analysis. Fluorescence in situ hybridization (FISH) analysis was performed on fixed bone marrow cells using IGH dual-color break-apart probe, CEBPE dual-color break-apart probe, whole chromosome paint (WCP) probe for chromosome 4, and Chromoprobe Multiprobe-ALL System probe. The 39-year-old female was diagnosed with B-ALL based on morphologic and immunophenotypic analyses. Conventional cytogenetic analysis showed a karyotype of 47, XX, +4, t(14;14) (q11;q32) [20], which was confirmed by FISH analysis. FISH using IGH-dual-color break-apart probe confirmed involvement of IGH gene in t(14;14) (q11;q32), and FISH using CEBPE dual-col