Hematopoiesis is normally maintained in osteoblast-specific Smad4 gene knockout mice

It was recently discovered that a subset of osteoblasts functions as a key component of the hematopoietic stem cells (HSC) niche in vivo, controlling HSC self-renewal and multi-lineage differentiation. Disruption of Smad4 gene specifically in osteoblasts leads to a remarkable decrease of osteoblast...

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Bibliographic Details
Published inZhongguo shi yan xue ye xue za zhi Vol. 16; no. 1; p. 159
Main Authors Lan, Yu, Tan, Xiao-Hong, Weng, Tu-Jun, Liu, Bing, Yang, Xiao
Format Journal Article
LanguageChinese
Published China 01.02.2008
Subjects
Animals
Bone Marrow Cells - cytology
Hematopoiesis
Hematopoietic Stem Cells - cytology
Hematopoietic Stem Cells - physiology
Mice
Mice, Knockout
Osteoblasts - cytology
Osteoblasts - physiology
Smad4 Protein - genetics
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Summary:It was recently discovered that a subset of osteoblasts functions as a key component of the hematopoietic stem cells (HSC) niche in vivo, controlling HSC self-renewal and multi-lineage differentiation. Disruption of Smad4 gene specifically in osteoblasts leads to a remarkable decrease of osteoblast number and endosteal surface area. In order to elucidate if the osteoblast loss has any effect on hematopoietic activity, the bone marrow (BM) and extramedullary hematopoiesis in the osteoblast-specific Smad4 knockout mice were systematically analyzed, the proportions of mature hematocytes in BM, liver and spleen were detected by flow cytometry, the hematopoietic progenitor number in different stages was measured by colong-forming assay, CFU-S and analysis of LSK cells. The results indicated that the conditional mutant mice demonstrated normal BM hematopoiesis without sign of extramedullary hematopoiesis. Furthermore, the proportion of hematopoietic progenitor cells was normal, while cell number/body weight of the
ISSN:1009-2137