Effects of hydrodynamics-mediated RNAi on Mfn2 expression, blood sugar and fat levels in mice

• Published in: Zhonghua gan zang bing za zhi Vol. 18; no. 10; p. 778
• Main Authors: Chen, Xiao-lin, Lei, You-rong
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2010
• Subjects: Animals; Blood Glucose - metabolism; Gene Expression; GTP Phosphohydrolases - genetics; GTP Phosphohydrolases - metabolism; Hydrodynamics; Lipids - blood; Liver - chemistry; Liver - metabolism; Male; Mice; Mice, Inbred BALB C; RNA Interference; RNA, Messenger - genetics
• ISSN: 1007-3418
• DOI: 10.3760/cma.j.issn.1007-3418.2010.10.013

To investigate the effects of hydrodynamics-mediated RNAi for Mfn2 gene expression in liver and the levels of blood sugar and fat in mice. Fifty-six male BALB/c mice were randomly divided into normal control group (NC, n = 8), negative control group (HK, n = 24) and transfection group (Mfn2, n = 24) according to random digits table. 1.5 ml plasmid (negative control or Mfn2 shRNA, 75mug for each mouse) diluted into phosphate buffered solution (PBS) was injected into the HK and Mfn2 groups mice via hydrodynamic intravascular injection. Mfn2 mRNA and protein expression in hepatic tissue was detected by RT-PCR and Western-blot 24 hours, 72 hours and 120 hours respectively after injection. At the same time, the levels of fasted blood sugar (FBS) and triglyceride (TG) were measured. Compared with HK mice, the expressions of Mfn2 mRNA (1.00+/-0.03 vs 1.14+/-0.07, t = 4.027, P = 0.007; 1.01+/-0.053 vs 1.18+/-0.07, t = 4.234, P = 0.006) and protein (7.81+/-0.80 vs 8.01+/-0.08, t = 2.941, P = 0.042; 8.05+/-0.15 vs 8.56