Experimental study on immune tolerance induced by human Ad-CTLA-4Ig in rats with pancreaticoduodenal transplantation

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 38; no. 3; p. 374
• Main Authors: Meng, Ling-xiang, Hu, Wei-ming, Zhang, Zhao-da, Han, Fang-hai, Zhou, Xiang, Jia, Wei, Lu, Hui-min
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2007
• Subjects: Adenoviridae - genetics; Animals; Antigens, CD - genetics; Blood Glucose - metabolism; CTLA-4 Antigen; Duodenum - immunology; Duodenum - metabolism; Duodenum - transplantation; Female; Gene Expression Regulation - immunology; Humans; Immune Tolerance - genetics; Immunoglobulins - genetics; Interleukin-2 - metabolism; Male; Pancreas - immunology; Pancreas - metabolism; Pancreas Transplantation - immunology; Rats; Survival Rate
• ISSN: 1672-173X

To study the immune tolerance induced by human Ad-CTLA-41g after pancreaticoduodenal transplantation of rats. Wistar rats with diabetes were induced by caudal intravenous administration of streptozotocin (STZ) at a single dose of 60 mg/kg. Pancreaticoduodenal transplantations were performed with the SD rats as donors and the diabete Wistar rats as recipients. The SD pancreaticoduodenal grafts with human Ad-CTLA-41g infection via arterial perfusion in vitro were transplanted into Wistar rats with diabetes. The blood glucose of the rats was measured after transplantation. The graft functional survival time (GFST) was recorded. The concentration of IL-2 was measured by ELISA on day 3 and day 10 after operation. The human CTLA-41g was detected by Western blot after operation. The GFST of control group without gene transfer was (11+/- 1) d, while that of gene transfer group was (33 +/-4) d (P< 0. 01). The expression of serum human CTLA-41g was detected in rats of gene transfer group on the 10th day after transplan