Optimization of microwave-assisted extraction with RSM and HPLC determination for three iridoid glycosides in Cornus officinalis

• Published in: Zhong yao cai Vol. 34; no. 7; p. 1118
• Main Authors: He, Juan-ni, Liu, Xiao, Mou, Zhao-li, Zhan, Han-ying, Zhang, Zhi-qi
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2011
• Subjects: Chromatography, High Pressure Liquid - methods; Cornus - chemistry; Fruit - chemistry; Glucosides - analysis; Glucosides - isolation & purification; Iridoid Glucosides - analysis; Iridoid Glucosides - isolation & purification; Iridoids - analysis; Iridoids - isolation & purification; Microwaves; Pyrans - analysis; Pyrans - isolation & purification; Quality Control; Reproducibility of Results; Solvents - chemistry
• ISSN: 1001-4454

To establish a method of microwave-assisted extraction and high performance liquid chromatographic (HPLC) for simultaneous determination of three iridoid glycosides including loganin, sweroside and cornuside in Cornus officinalis. The extraction conditions of microwave power,ethanol concentration, liquid to sample ratio were optimized with a response surface methodology (RSM); Three constituents were separated on an Agilent TC-C18 column by gradient elution using acetonitrile and water as the mobile phase. The flow rate was 1.0 mL/min. The detection wave length was 240 nm. The optimal conditions of microwave extraction were as follows: microwave power 400 W, ethanol concentration 72%, liquid to sample ratio 15 mL/g, the extraction time 10 min, the extraction times 2; The HPLC peak areas of all the constituents showed good linearity (r>0.9994) in the range of the tested concentration,the average recoveries of the method were 98.68%, 98.24% and 98.29%, respectively. The established method of microwave-assisted