Effect of HIF-1α and its mechanism on invasion and metastasis of esophageal carcinoma Eca109 cells in vitro and in vivo

• Published in: Xi bao yu fen zi mian yi xue za zhi Vol. 28; no. 1; p. 67
• Main Authors: Jing, Shao-wu, Wang, Ya-di, Zheng, Ming-min, Sun, Guo-gui, Liu, Qing, Cheng, Yun-jie, Yang, Cong-rong
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2012
• Subjects: Animals; Cadherins - genetics; Cadherins - metabolism; Cell Hypoxia - genetics; Cell Line, Tumor; Esophageal Neoplasms - genetics; Esophageal Neoplasms - metabolism; Esophageal Neoplasms - pathology; Female; Humans; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 2 - metabolism; Mice; Mice, Nude; Neoplasm Invasiveness - genetics; Neoplasm Metastasis - genetics; RNA Interference; RNA, Messenger - metabolism; Transplantation, Heterologous
• ISSN: 1007-8738

To investigate the effects of HIF-1α by RNAi on invasion and metastasis of esophageal carcinoma Eca109 cells in vitro and in vivo, in order to explore its probable mechanism. CoCl(2); was used to mimic tumor hypoxic microenvironment. mRNA and protein levels of HIF-1α, E-cadherin and MMP-2 under hypoxia were detected by RT-PCR and immunohistochemistry. The effects of silencing HIF-1α by RNAi on HIF-1α, E-cadherin and MMP-2 mRNA were detected by RT-PCR. The effect of RNAi on invasion and metastasis were tested by cell scratch assay and transwell chambers. The Eca109-implanted nude mouse model was established, and the effects of HIF-1αon tumor growth and lymphoid node metastasis were observed. The expressions of HIF-1α, E-cadherin and MMP-2 in transplanted tumors were detected by Western blot, and the effects of HIF-1α on tumor growth, invasion and metastasis in vitro and in vivo were analyzed. Hypoxia up-regulated HIF-1α protein, mRNA and protein levels of E-cadherin down-regulated, and MMP-2 up-regulated, whil