Effect of Guanxinkang on ATP-sensitive potassium channel in myocardial cells of rat with ischemic/ reperfusion injury

• Published in: Zhongguo Zhong xi yi jie he za zhi Vol. 30; no. 11; p. 1186
• Main Authors: Zhang, Na, Chen, Eu-Rang, Zhang, Yi-Yi
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2010
• Subjects: Animals; Calcium - metabolism; Drugs, Chinese Herbal - pharmacology; KATP Channels - drug effects; Male; Myocardial Ischemia - metabolism; Myocardial Ischemia - physiopathology; Myocardial Reperfusion Injury - prevention & control; Myocytes, Cardiac - metabolism; Rats; Rats, Wistar
• ISSN: 1003-5370

OBJECTIVE To investigate the effect of Guanxinkang (GXK) on ATP-sensitive potassium channel in myocardial cells of rat with ischemic/reperfusion injury and its possible mechanism for cardiac vascular protection and anti-myocardial ischemia. Wistar rats were established into I/R injured models by 10 min perfusion--30 min no-flow ischemia--45 min reperfusion, and divided into 5 groups: the I/R model group and 4 treatment groups treated respectively with glibenclamide, pinacidil, GXK and GXK+glibenclamide. Rats' heart were isolated for detecting Ca(2+)-Mg(2+)-ATPase, Na(+)-K(+)-ATPase activity in myocardial cells, and the changes of current in ATP-sensive potassium channel (K(ATP)) was recorded by whole patch clamp technique. Data were controlled by those taken from normal rats in a control group. K(ATP) in the GXK treated group were higher than that in the I/R model group; and similar to that in the pinacidil treated group (P > 0.05). As compared with the model group, activities of Ca(2+)-Mg(2+)-ATPase and Na(+