Preparation and biological characteristics of a new doxorubicin-gelatin-microspheres for hepatic artery embolization

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 42; no. 1; p. 119
• Main Authors: Wan, Yi, Ma, Yu, Chen, Xiao-li, Duan, Li-geng, Luo, Dong-hui, Xia, Chuan-qin
• Format: Journal Article
• Language: Chinese
• Published: China 01.01.2011
• Subjects: Antibiotics, Antineoplastic - administration & dosage; Carcinoma, Hepatocellular - therapy; Chemoembolization, Therapeutic - methods; Cross-Linking Reagents - chemistry; Dextrans - chemistry; Doxorubicin - administration & dosage; Gelatin - administration & dosage; Hepatic Artery; Humans; Liver Neoplasms - therapy; Microspheres
• ISSN: 1672-173X

To prepare a new doxorubicin-gelatin-microspheres (DR-GMs) suitable for hepatic artery chemoembolization. Oxidized dextran and glutaraldehyde were used respectively as crosslinking agent for preparing DR-GMs. Orthogonal design was employed to optimize the preparation of the oxidized dextran cross-linked GMs. The morphology, swelling, and in vitro and in vivo degrading were compared between the two groups of microspheres, both with 60% degree of cross-linking. The granulometers of both groups of microspheres fitted for hepatic artery embolization. The roundness of the microspheres (observed with scanning electron microscope) crosslinked by oxidized-dextran was better than those crosslinked by glutaraldehyde. The microspheres crosslinked by oxidized-dextran had an average diameter of (78.2 +/- 8.1) microm, and a narrow size distribution (76.4 +/- 3.2)%, which ranged from 50 to 125 microm. The drug content rate and encapsulation rate of the microspheres crosslinked by oxidized-dextran were (87.5 +/- 0.9)% and (1