Effects of matrine on invasion and metastasis of leukemia cell line Jurkat

• Published in: Zhongguo Zhong xi yi jie he za zhi Vol. 28; no. 10; p. 907
• Main Authors: Zhang, Wei, Dai, Bi-Tao, Xu, You-Hua
• Format: Journal Article
• Language: Chinese
• Published: China 01.10.2008
• Subjects: Alkaloids - pharmacology; Animals; Cell Proliferation - drug effects; Down-Regulation; Drugs, Chinese Herbal - pharmacology; Humans; Jurkat Cells; Leukemia - drug therapy; Leukemia - pathology; Leukemia - physiopathology; Mice; Neoplasm Invasiveness; Neoplasm Metastasis; NIH 3T3 Cells; Quinolizines - pharmacology; Random Allocation
• ISSN: 1003-5370

To study the inhibitory effects of matrine, in different concentrations, on invasion and metastasis of human acute lymphocytic leukemia cell line Jurkat. In vitro cultured Jurkat cells were treated by matrine in concentration of 0 g/L, 0.1 g/L, 0.15 g/L and 0.2 g/L, respectively. Then cell adhesion assay, cell migration assay and matrigel invasion assay were used respectively to observe the effects of matrine on adhesion, migration and invasive capacity of Jurkat cells. Meantime, RT-PCR was performed to detect the matrix metalloproteinase (MMP)-2 and MMP-9 mRNA expression levels. Comparison of measurement data among groups was analyzed by variance analysis. As compared with the control group, the adhesion of Jurkat to fibronectin (FN) was significantly inhibited by 0.15 g/L and 0.2 g/L of matrine (P < 0.05); the cell migration and invasive capacity were significantly lowered by 0.1 g/L, 0.15 g/L and 0.2 g/L matrine (P < 0.01). High mRNA expression of MMP-9 presented but that of MMP-2 was expressed insignifica