Transplantation of porcine type I atelocollagen into rabbit cornea

• Published in: Chung-hua yen k'o tsa chih Vol. 47; no. 5; p. 451
• Main Authors: Wang, Lin-ni, Rong, Hui-feng, Yan, Hua, Liang, Shuang, Wang, Peng-fei, Liu, Wen-guang
• Format: Journal Article
• Language: Chinese
• Published: China 01.05.2011
• Subjects: Animals; Collagen; Corneal Transplantation - methods; Rabbits; Swine; Transplantation, Heterologous; Wound Healing
• ISSN: 0412-4081
• DOI: 10.3760/cma.j.issn.0412-4081.2011.05.013

To investigate corneal wound healing in rabbit after lamellar keratoplasty with porcine type I atelocollagen. One hundred Chinese white rabbits, 80 rabbits were randomly divided into collagen materials group (group A) and allograft group (group B), and group A and B were operated for the lamellar keratoplasty in one eye. Rest 20 rabbits' eyes (40 eyes) were used as the source of allograft for group B. After operation, the rabbits' eyes were observed by naked eyes and slit lamp, and recorded the score of the transparency and neovascularization of the cornea. The rabbits were sacrificed at 3rd, 14th day, the 1st, 3rd, and 6th month (each group eight eyes). The corneas were observed by histopathology, and the epithelial cells marker protein K3 were detected by immunohistochemistry. The results were analyzed by Rank sum test. The transparency of the cornea was increased gradually in A and B groups, and reached the peak on the 6th month (group A: χ(2) = 31.250, P = 0.000; group B: χ(2) = 32.566, P = 0.000). The di