Genotyping of major outer membrane protein gene of Chlamydia trachomatis by cleavase fragment length polymorphism analysis

To establish a methed of cleavage fragment length polymorphism (CFLP) analysis with a primer labeled at the 5'-end with digoxigenin for genotyping of Chlamydia trachomatis (Ct). The methods for detection of Ct by major outer membrane protein (MOMP) gene (ompl) with nested polymerase chain react...

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Published inZhonghua er ke za zhi Vol. 43; no. 1; p. 5
Main Authors Zhong, Xiao-Yun, Yu, Jia-Lin, Wang, Jia, Deng, Bing, Liu, Guan-Xin, Yu, You-Xia, Wang, Chao-Hui, Zhang, Yu, Li, Yi
Format Journal Article
LanguageChinese
Published China 01.01.2005
Subjects
Cervix Uteri - microbiology
Chlamydia Infections - diagnosis
Chlamydia trachomatis - genetics
DNA Primers
Female
Genes, Bacterial - genetics
Genotype
Humans
Polymerase Chain Reaction
Polymorphism, Genetic
Pregnancy
Pregnancy Complications, Infectious - diagnosis
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Summary:To establish a methed of cleavage fragment length polymorphism (CFLP) analysis with a primer labeled at the 5'-end with digoxigenin for genotyping of Chlamydia trachomatis (Ct). The methods for detection of Ct by major outer membrane protein (MOMP) gene (ompl) with nested polymerase chain reaction (ompl-nPCR) were studied. The incidence of Ct infection in pregnant women, the common genotypes and vertical transmission rate of Ct in Chongqing area during the past one year was also investigated. The samples were taken from cervical scrapes of parturient women and nasopharygeal swabs of their neonates from April 2003 to Feb. 2004 in Chongqing Women and Children's Health Care Institute. Totally 300 pairs (605 specimens) were detected by using ompl-nPCR, ompl-PCR (inside pair of primers was used directly) and plasmid-PCR. The results were judged by the modified gold standard (MGS). The ompl-nPCR amplified DNA was purified by recovery of DNA from agarose gel electroelution into dialysis bags. The DNA amplified from
ISSN:0578-1310