Studies on the killing effect of gamma ray irradiated peripheral blood mononuclear cells combined with (-)-gossypol on human prostate cancer LNCaP cells

To observe the killing effect of irradiated peripheral blood mononuclear cells (PBMCs) at low dose combined with (-)-gossypol on cultured human prostate cancer LNCaP cells. Human PBMCs were irradiated by gamma ray at 1 gray, the irradiation dose rate was 17 Gy/min. The experiments were divided into...

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Published inXi bao yu fen zi mian yi xue za zhi Vol. 26; no. 11; p. 1089
Main Authors Zhan, Wen-hua, Zhan, Yu-xiang, Yan, Gang, Zhang, Xian-qing
Format Journal Article
LanguageChinese
Published China 01.11.2010
Subjects
Cell Line, Tumor
Gamma Rays
Gossypol - pharmacology
Humans
Leukocytes, Mononuclear - immunology
Leukocytes, Mononuclear - radiation effects
Male
Prostatic Neoplasms - drug therapy
Prostatic Neoplasms - pathology
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Summary:To observe the killing effect of irradiated peripheral blood mononuclear cells (PBMCs) at low dose combined with (-)-gossypol on cultured human prostate cancer LNCaP cells. Human PBMCs were irradiated by gamma ray at 1 gray, the irradiation dose rate was 17 Gy/min. The experiments were divided into control group (LNCaP cells only), LNCaP cells with irradiated and non-irradiated PBMCs co-culture groups, (-)-gossypol treatment group, irradiated PBMCs and (-)-gossypol co-treatment group. Acridine orange/ethidium bromide (AO/EB) staining and MTT method were used to observe the killing effect of PBMCs and/or (-)-gossypol. The killing activity of irradiated PBMCs group and (-)-gossypol treatment group were obviously increased and were higher than that of non-irradiated group (P < 0.05). The killing activity of combined group was much higher than that of irradiated group and ApoG2 treatment group (P < 0.01). Irradiated PBMCs at low dose combined with (-)-gossypol can enhance the anti-tumor effects obviously.
ISSN:1007-8738