The labeling of procollagen gene probe by PCR and the detection of procollagen gene expression in hepatic stellate cells

To establish a new method for labeling of procollagen gene probe and the detection of procollagen mRNA expression in HSC. According to gene sequences from NCBI Gene Bank, the primers for the amplification of type I, III and IV procollagen genes were designed by OLIGO software, the procollagen genes...

Full description

Saved in:
Bibliographic Details
Published inZhongguo ying yong sheng li xue za zhi Vol. 17; no. 3; p. 305
Main Authors Zhao, W X, Liang, C L, Zhao, B, Pang, R Q, Chen, Z L
Format Journal Article
LanguageChinese
Published China 01.08.2001
Subjects
Cells, Cultured
DNA Probes
Gene Expression
Hepatic Stellate Cells - metabolism
Humans
Polymerase Chain Reaction - methods
Procollagen - genetics
Online AccessGet more information

Cover

More Information
Summary:To establish a new method for labeling of procollagen gene probe and the detection of procollagen mRNA expression in HSC. According to gene sequences from NCBI Gene Bank, the primers for the amplification of type I, III and IV procollagen genes were designed by OLIGO software, the procollagen genes were amplified by RT-PCR and were labeled by PCR and DIG-dUTP, and the probes were applied to detect procollagen mRNA expression in cultured hepatic stellate cells by in situ hybridization. The procollagen genes were successfully amplified by the Primers and RT-PCR and were labeled by DIG-dUTP and PCR, the procollagen mRNA expression in cultured hepatic stellate cells was detected by the probes. A new simplified method for labeling of procollagen genes is successfully found and it can be used for other gene amplification and labeling.
ISSN:1000-6834