Identification and expression analysis of Macaca mulatta piwil4 gene

To investigate the structure and expression pattern of rhesus monkey PIWIL4 protein, homologous comparison and reverse transcription PCR (RT-PCR) were carried out to identify rhesus monkey piwil4. The expression of piwil4 mRNA was tested in rhesus monkey heart, brain, colon, epididymis and testis, a...

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Published inYíchuán Vol. 33; no. 4; p. 365
Main Authors Zhao, Shao-Zhi, Li, Yue, Jiang, Xue, Lu, Yi-Lu, Tao, Da-Chang, Liu, Yun-Qiang, Ma, Yong-Xin
Format Journal Article
LanguageChinese
Published China 01.04.2011
Subjects
Amino Acid Sequence
Animals
Argonaute Proteins
Humans
Immunohistochemistry
Macaca mulatta - genetics
Male
Molecular Sequence Data
Proteins - analysis
Proteins - genetics
Testis - chemistry
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Summary:To investigate the structure and expression pattern of rhesus monkey PIWIL4 protein, homologous comparison and reverse transcription PCR (RT-PCR) were carried out to identify rhesus monkey piwil4. The expression of piwil4 mRNA was tested in rhesus monkey heart, brain, colon, epididymis and testis, and the result showed that piwil4 mRNA was expressed in these rhesus monkey tissues. Bioinformatic analysis suggested that the rhesus PIWIL4 protein shared 97% identity in amino acids and the same domains such as PAZ and Piwi with the human PIWIL4 (HIWI2) protein. The immunohistochemical result indicated that PIWIL4 proteins had the same localization in adult testes of the two species, but the distribution of these proteins was altered dynamically at different developmental stages in rhesus monkey testes. PIWIL4 protein was expressed in the nucleus of convoluted seminiferous tubules in infant monkey testes, whereas it was expressed in the cytoplasm of adult monkey testes. The results suggest that piwil4 gene play a
ISSN:0253-9772
DOI:10.3724/SP.J.1005.2011.00365