The observation of engraftment of human umbilical cord blood-derived hematopoietic stem/progenitor cells in xenotransplanted NOD/SCID mouse model by intra-bone marrow injection

To explore whether intra-bone marrow injection strategy could promote the engraftment of human umbilical cord blood derived hematopoietic stem/progenitor cells (HS/PC) in xenotransplanted NOD/SCID mouse model. Aliquots containing 1 x 10(3), 1 x 10(4), 0.5 x 10(5), 1 x 10(5) and 5 x 10(5) human umbil...

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Published inZhōnghuá xuèyèxué zázhì Vol. 29; no. 6; p. 361
Main Authors Gao, Jing-tao, Lu, Shin-hong, Li, Yan-han, Yang, Zhou, Xu, Jing, Zheng, Yi-zhou
Format Journal Article
LanguageChinese
Published China 01.06.2008
Subjects
Animals
Antigens, CD34
Bone Marrow
Cord Blood Stem Cell Transplantation - methods
Female
Fetal Blood - cytology
Hematopoietic Stem Cells - cytology
Humans
Mice
Mice, Inbred NOD
Mice, SCID
Transplantation Conditioning
Transplantation, Heterologous
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Summary:To explore whether intra-bone marrow injection strategy could promote the engraftment of human umbilical cord blood derived hematopoietic stem/progenitor cells (HS/PC) in xenotransplanted NOD/SCID mouse model. Aliquots containing 1 x 10(3), 1 x 10(4), 0.5 x 10(5), 1 x 10(5) and 5 x 10(5) human umbilical cord blood (hUCB) CD34+ cells were transplanted into sublethally irradiated NOD/SCID mice via intra-venous (i.v.) and intra-bone marrow (iBM) injection. The homing and long-term engraftment capabilities of hUCB CD34+ cells from right tibia, right femur, left tibia, left femur and spleen were detected by PCR 24h after xenotransplantation and by FACS 8-week after xenotransplantation. Tissues of liver, spleen, lungs, or cells from peripheral blood, right tibia, right femur, left tibia and left femur 24 hours after xenotransplantation in iBM injecting 5 x 10(5) CD34+ cells recipients expressed human chromosome 17 specific alpha-satellite fragment. 8-week engraftment of human cells was observed and engraftment leve
ISSN:0253-2727