Effects of rapamycin-loaded poly(lactic-co-glycolic) acid nanoparticles on distribution of cell cycle, expression of p27 protein, and proliferation of human umbilical arterial vascular smooth muscle cell in vitro

To evaluate the effects of rapamycin (RPM)-loaded poly (lactic-co- glycolic) acid (PLGA) nanoparticles (NPs) on the proliferation, distribution of cell cycle, and expression of p27 protein in human umbilical arterial vascular smooth muscle cell (HUASMC) in vitro. The primarily culture model of HUASM...

Full description

Saved in:
Bibliographic Details
Published inZhōngguó yīxué kēxuéyuàn xuébào Vol. 32; no. 1; p. 32
Main Authors Miao, Li-fu, Huang, Chao-lian, Chen, Lian-feng, Zhu, Wen-ling, Yang, Jing, Wang, Yi-guang, Zhang, Hua, Liu, Pei-mao, She, Ming-peng, Song, Cun-xian
Format Journal Article
LanguageChinese
Published China 01.02.2010
Subjects
Cell Cycle - drug effects
Cell Proliferation - drug effects
Cells, Cultured
Cyclin-Dependent Kinase Inhibitor p27 - metabolism
Drug Carriers
Humans
Lactic Acid
Muscle, Smooth, Vascular - cytology
Myocytes, Smooth Muscle - cytology
Myocytes, Smooth Muscle - drug effects
Myocytes, Smooth Muscle - metabolism
Nanoparticles
Polyglycolic Acid
Sirolimus - administration & dosage
Sirolimus - pharmacology
Umbilical Arteries - cytology
Online AccessGet more information

Cover

More Information
Summary:To evaluate the effects of rapamycin (RPM)-loaded poly (lactic-co- glycolic) acid (PLGA) nanoparticles (NPs) on the proliferation, distribution of cell cycle, and expression of p27 protein in human umbilical arterial vascular smooth muscle cell (HUASMC) in vitro. The primarily culture model of HUASMC was successfully established by explant-attached method in vitro. The cells were administrated with different doses of RPM, and RPM-PLGA NPs were observed as treat groups compared with PLGA NPs and M231-SMGs medium cultured group. The effect of RPM-PLGA NPs on proliferation of HUASMC was assessed using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) colorimetry method. The influences of RPM-PLGA NPs on the cell cycle and cellular growth kinetics of HUASMCs were tested by flow cytometry. The effect of RPM-PLGA NPs on the expression of p27 protein of HUASMCs was assessed through an immunohistochemical method. Compared with the control group, the proliferation of HUASMCs was inhibited by 50 micro
ISSN:1000-503X
DOI:10.3881/j.issn.1000-503X.2010.01.010