Effect of toluene diisocyanate on reactive oxygen species production and permeability of human bronchial epithelial cells in vitro

• Published in: Nan fang yi ke da xue xue bao = Journal of Southern Medical University Vol. 31; no. 2; p. 239
• Main Authors: Mo, Guan-wen, Cai, Shao-xi, Zhao, Hai-jin, Li, Wen-jun, Tong, Wan-cheng, Liu, Lai-yu
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2011
• Subjects: Bronchi - cytology; Cell Line; Cell Membrane Permeability - drug effects; Epithelial Cells - cytology; Epithelial Cells - metabolism; Humans; Oxidative Stress - drug effects; Reactive Oxygen Species - metabolism; Serum Albumin - pharmacology; Toluene 2,4-Diisocyanate - pharmacology
• ISSN: 1673-4254

To investigate the effect of toluene diisocyanate (TDI) on the production of reactive oxygen species (ROS) and the permeability of human bronchial epithelial (HBE) cells. TDI-human serum albumin (TDI-HSA) conjugate was prepared using a modified Son's method. MTT assay was used to assess HBE cell viability after exposure to different concentrations of TDI-HSA. The level of intracellular ROS of HBE cells was detected by flow cytometry with an oxidation-sensitive fluorescent probe 2',7'-dichlorofluorescein diacetate (DCFH-DA) uploading, and the permeability of cell monolayer was assessed by detecting the transepithelial electrical resistance (TEER). The exposure to 120 µg/ml TDI-HSA did not obviously affect the cell viability. Compared with the control group, the intracellular fluorescent intensity increased significantly in the cells exposed to 20, 60, and 100 µg/ml TDI-HSA (P<0.05). The intracellular ROS production increased significantly after 100 µg/ml TDI-HSA treatment (P<0.05), but the increment in ROS pro