Inhibition of ACh on the delayed rectifier-like potassium current in acutely isolated cerebral cortical neurons of rats

The modulation of ACh on delayed rectifier-like potassium currents (I(K)) was studied in freshly dissociated cerebral cortical neurons using the whole-cell patch-clamp technique. Wistar rats between 10- and 14-day old of both sexes were used. After rats were decapitated, their brains were quickly re...

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Published inSheng li hsüeh pao Vol. 58; no. 1; p. 58
Main Authors Cui, Lan-Wei, Li, Yu-Rong, Yang, Lei, Jia, Shu-Wei, Qu, Li-Hui, Yao, Kun, Jin, Hong-Bo
Format Journal Article
LanguageChinese
Published China 25.02.2006
Subjects
Acetylcholine - physiology
Animals
Cell Separation
Delayed Rectifier Potassium Channels - antagonists & inhibitors
Female
Male
Neurons - metabolism
Neurons - physiology
Patch-Clamp Techniques
Protein Kinase C - metabolism
Protein Kinase C - physiology
Rats
Rats, Wistar
Receptor, Muscarinic M3 - metabolism
Receptors, Nicotinic - metabolism
Signal Transduction - physiology
Somatosensory Cortex - cytology
Somatosensory Cortex - physiology
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Summary:The modulation of ACh on delayed rectifier-like potassium currents (I(K)) was studied in freshly dissociated cerebral cortical neurons using the whole-cell patch-clamp technique. Wistar rats between 10- and 14-day old of both sexes were used. After rats were decapitated, their brains were quickly removed, iced, and then manually cut into 400 mum slices. Slices were then incubated for 0.5 h at 32 degrees C in a buffered artificial cerebrospinal fluid (ACSF) bubbled with 95% O2, 5% CO2. Slices were then removed into buffered ACSF containing protease (0.5 mg/ml) at 32 degrees C. After 30 min of enzyme digestion, tissue was rinsed three times in the buffered saline. Then the enzyme-treated slices were mechanically dissociated with a graded series of fire-polished Pasteur pipettes. The cell suspension was then plated into a 35 mm dish and placed on the stage of a Olympus inverted microscope. For whole-cell recordings of currents, standard voltage-clamp techniques were used. Neurons were held at -80 mV, and the I(K
ISSN:0371-0874