Cloning and expression analysis of ZFP207 encoding a TFA-type zinc finger protein in rice

• Published in: Yíchuán Vol. 32; no. 4; p. 387
• Main Authors: Meng, Fan-Jun, Huang, Ji, Bao, Yong-Mei, Jiang, Yan, Zhang, Hong-Sheng
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2010
• Subjects: Amino Acid Sequence; Cloning, Molecular; Gene Expression Regulation, Plant; Molecular Sequence Data; Oryza - genetics; Phylogeny; Plant Proteins - chemistry; Plant Proteins - classification; Plant Proteins - genetics; Plant Proteins - metabolism; Sequence Analysis, DNA; Transcription Factor TFIIIA - chemistry; Transcription Factor TFIIIA - classification; Transcription Factor TFIIIA - genetics; Transcription Factor TFIIIA - metabolism; Transcriptional Activation; Zinc Fingers
• ISSN: 0253-9772
• DOI: 10.3724/SP.J.1005.2010.00387

The zinc finer proteins consist of a large transcription factor family involved in plant development and responses to environmental stresses. In this paper, a TFA-type zinc finger protein gene ZFP207 (GenBank assession number AK063147.1) was cloned from rice variety Jiucaiqing by RT-PCR approach. This gene contains an open reading frame (ORF) of 567 bp, which encodes a peptide of 188 amino acid residues. The isoelectric point (pI) of the protein is 9.67, and its molecular weight is 20.72 kDa. Bioinformatic analysis showed that the ZFP207 protein comprises a typical TFA-type zinc finger domain and an EAR-motif at its C-terminus. However, nuclear localization signals (NLS) commonly existing in TFA-type zinc finger proteins was not found in the ZFP207 amino acid sequence. In addition, based on the alignments of the whole amino acid sequences of some known TFA-type zinc finger proteins in plants, a phylogenetic tree was con-structed by the neighbour joining method. The phylogenetic tree showed that ZFP207 and oth