Bortezomib-induced BiP expression and apoptosis in multiple myeloma cells

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 17; no. 1; p. 107
• Main Authors: Dong, Hong-Juan, Chen, Xie-Qun, Gao, Guang-Xun, Gu, Hong-Tao, Pan, Yao-Zhu, Gao, Ying, Zhu, Hua-Feng
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2009
• Subjects: Apoptosis - drug effects; Boronic Acids - pharmacology; Bortezomib; Cell Line, Tumor; Endoplasmic Reticulum - drug effects; Endoplasmic Reticulum - metabolism; Heat-Shock Proteins - metabolism; Humans; Multiple Myeloma - metabolism; Multiple Myeloma - pathology; Pyrazines - pharmacology; RNA, Messenger - genetics
• ISSN: 1009-2137

This study was aimed to explore the effect of bortezomib on the apoptosis and expression of the molecular chaperone BiP in human multiple myeloma cell line NCI-H929 (H929). After treatment of H929 cells with different concentrations of bortezomib for 24 hours, cell apoptosis was assayed by flow cytometry with Annexin V-FITC/PI staining, and the expression levels of BiP mRNA and protein were detected by RT-PCR and Western blotting analysis. The results showed that bortezomib of different concentrations (20, 40 and 80 nmol/L) induced apoptosis of H929 cells in dose-dependent manner, with apoptotic rates (15.73 +/- 0.67)%, (27.83 +/- 1.26)% and (44.17 +/- 2.25)% respectively, which were significantly higher than that in control (1.21 +/- 0.07%) (p < 0.05). Bortezomib-induced up-regulation of BiP mRNA levels was almost on a parallel with BiP protein when compared with control. Under the similar apoptosis-stimulating conditions with apoptotic rates varying from 40% to 50%, expression levels of BiP mRNA and BiP pro