Identification and characterization of a purple sulfur bacterium from mangrove with rhodopin as predominant carotenoid

To exploit resources of purple sulfur bacteria in China and further investigate its response mechanism to ecological environment of mangrove. Repeated agar shake dilution method, microscope techniques, UV-Vis absorption spectra, thin layer chromatography, HPLC and MS were used. We isolated a purple...

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Bibliographic Details
Published inWei sheng wu hsüeh pao Vol. 51; no. 10; p. 1318
Main Authors Zhao, Jiangyan, Fu, Yingnan, Zhao, Chungui, Yang, Suping, Qu, Yinbo, Jiao, Nianzhi
Format Journal Article
LanguageChinese
Published China 01.10.2011
Subjects
Bacteriochlorophyll A - metabolism
Carotenoids - biosynthesis
Chromatiaceae - classification
Chromatiaceae - genetics
Chromatiaceae - isolation & purification
Chromatiaceae - metabolism
DNA, Ribosomal - genetics
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S - genetics
Seawater - microbiology
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Summary:To exploit resources of purple sulfur bacteria in China and further investigate its response mechanism to ecological environment of mangrove. Repeated agar shake dilution method, microscope techniques, UV-Vis absorption spectra, thin layer chromatography, HPLC and MS were used. We isolated a purple sulfur bacterium, designated as strain YL28, from a intertidal sediment sample collected from inshore mangrove near Luoyang Bridge of Quanzhou city, Fujian Province of China. Cells were ovoid to rod shaped, 0.5 microm - 1 microm x 2 microm - 3 microm. Color of cell suspensions was reddish-brown. It possessed vesicular intracytoplasmic membrane structures, contained rhodopin and phytylated bacteriochlorophyll a as well as the other two novel bacteriochlorophyll a intermediates. The optimum growth was at 2% - 3.5% NaCl, pH 5.7 - 6.7 and 20 degrees C - 35 degrees C. Photoautotrophically growth anaerobically in the light with sulphide, sulphur, thiosulfate, sulfite as electron donor. Globules of S(0) distributed inside
ISSN:0001-6209