Identification and characterization of a purple sulfur bacterium from mangrove with rhodopin as predominant carotenoid
To exploit resources of purple sulfur bacteria in China and further investigate its response mechanism to ecological environment of mangrove. Repeated agar shake dilution method, microscope techniques, UV-Vis absorption spectra, thin layer chromatography, HPLC and MS were used. We isolated a purple...
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Published in | Wei sheng wu hsüeh pao Vol. 51; no. 10; p. 1318 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | Chinese |
Published |
China
01.10.2011
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Subjects | |
Online Access | Get more information |
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Summary: | To exploit resources of purple sulfur bacteria in China and further investigate its response mechanism to ecological environment of mangrove.
Repeated agar shake dilution method, microscope techniques, UV-Vis absorption spectra, thin layer chromatography, HPLC and MS were used.
We isolated a purple sulfur bacterium, designated as strain YL28, from a intertidal sediment sample collected from inshore mangrove near Luoyang Bridge of Quanzhou city, Fujian Province of China. Cells were ovoid to rod shaped, 0.5 microm - 1 microm x 2 microm - 3 microm. Color of cell suspensions was reddish-brown. It possessed vesicular intracytoplasmic membrane structures, contained rhodopin and phytylated bacteriochlorophyll a as well as the other two novel bacteriochlorophyll a intermediates. The optimum growth was at 2% - 3.5% NaCl, pH 5.7 - 6.7 and 20 degrees C - 35 degrees C. Photoautotrophically growth anaerobically in the light with sulphide, sulphur, thiosulfate, sulfite as electron donor. Globules of S(0) distributed inside |
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ISSN: | 0001-6209 |