Inhalation of glucocorticosteroid in the rat model of bleomycin-induced pulmonary fibrosis and its impact on the expression of basic fibroblast growth factor

• Published in: Zhongguo ying yong sheng li xue za zhi Vol. 26; no. 3; p. 330
• Main Authors: Mu, Jian-Qin, Li, Guo-Qiang, Wei, Lu-Qing
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2010
• Subjects: Administration, Inhalation; Animals; Fibroblast Growth Factor 2 - metabolism; Glucocorticoids - administration & dosage; Glucocorticoids - therapeutic use; Male; Pulmonary Fibrosis - drug therapy; Pulmonary Fibrosis - metabolism; Pulmonary Fibrosis - pathology; Rats; Rats, Wistar
• ISSN: 1000-6834

To study the effect of inhalation of glucocorticoid on bleomycin-induced pulmonary fibrosis in rats and its possible mechanism. Pulmonary fibrosis was induced in Wistar rats by intratracheal instillation of bleomycin (group M, group B, group D). Group B inhaled glucocorticoid daily from the next day of received bleomycin. Group D intraperitoneal injection glucocorticoid daily from the next day of received bleomycin. Normal controls received normal saline both intratracheally. Five rats in each group were killed at 1, 4 week after intratracheal instillation. Histological changes of the lungs were evaluated by HE, Masson trichrome stain. Lung expression of bFGF proteins was assessed by immunohistochemistry and the level of bFGF protein in serum and BALF was further measured by ELISA. Pulmonary fibrosis of group M was higher than that of group C, pulmonary fibrosis of group B, D was lower than that of group M at 1, 4 week. bFGF in group M was higher than that in group C, bFGF in group B, D was lower than that in