Inhibitive effect of artesunate on human lymphoblastic leukemia/lymphoma cells

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban Vol. 40; no. 6; p. 1038
• Main Authors: Zeng, Yan, Ni, Xun, Meng, Wen-Tong, Wen, Qin, Jia, Yong-Qian
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2009
• Subjects: Antineoplastic Agents, Phytogenic - pharmacology; Apoptosis - drug effects; Artemisinins - pharmacology; Blood-Brain Barrier - drug effects; Cell Line, Tumor; Cytarabine - pharmacology; Drug Synergism; Humans; Jurkat Cells; Lymphoma - pathology; Precursor Cell Lymphoblastic Leukemia-Lymphoma - pathology
• ISSN: 1672-173X

To test the effect of Artesunate (ART) on the proliferation of Raji cells, Jurkat cells and acute lymphoblastic leukemia (ALL) primary cells; to determine the synergistic antiproliferation effect between ART and Vincristine (VCR) or Cytarabine(Ara-C) on Raji and Jurkat cells; and to explore the mechanism of ART induced apoptosis of tumor cells in vitro. MTT assay was performed to detect the inhibition of proliferation of Raji, Jurkat, and ALL primary cells. The cells were exposed to ART at various concentrations with or without VCR or Ara-C. The morphological changes of Raji and Jurkat cells were observed under light microscopy after Wright-Giemsa dyeing and electron transmission microscopy. The mitochondria transmenbrane potential was measured by Rhodamine 123 staining. Colorimetric method was used to measure the activities of caspase-3 in those tumor cells. ART inhibited the proliferation of Raji cells, Jurkat cells and ALL primary cells. The cytotoxicity of ART on Raji cells and Jurkat cells at a low conce