Influence of electroacupuncture plus intragastric administration of extract of hypericum perforatum L on ethology and brain microcirculation in depression rats

• Published in: Zhēncì yánjiū Vol. 36; no. 3; p. 164
• Main Authors: Yu, Bin, Sun, Yong, Lü, Gao-Hong, Lu, Jin-Fu, Fang, Tai-Hui
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2011
• Subjects: Administration, Topical; Animals; Brain - blood supply; Brain - drug effects; Combined Modality Therapy; Depression - drug therapy; Depression - physiopathology; Depression - psychology; Depression - therapy; Disease Models, Animal; Drugs, Chinese Herbal - administration & dosage; Electroacupuncture; Ethology; Female; Humans; Hypericum - chemistry; Microcirculation - drug effects; Random Allocation; Rats; Rats, Sprague-Dawley
• ISSN: 1000-0607

To observe the effect of combined administration of intragastric perfusion of extract of Hypericum Perforatum L (HP-L) and electroacupuncture (EA) of "Baihui" (GV 20) and "Yamen" (GV 15) on behavior and brain microcirculation in depression rats. Female SD rats were randomized into control, model, lower-dose of HP-L (lower-dose in short, 10 mg/kg), lower-dose+ EA, higher-dose (20 mg/kg) and higher-dose+ EA groups (n = 10/group). Depression model was established by lonely raising and chronic unpredictable mild stress (tail cramping, water-deprivation, fasting, electrical shock stimulation, etc. ) for 21 days. EA (2 Hz, 1 mA) was applied to "Baihui"(GV 20) and "Yamen"(GV 15) for 20 min, once daily for 14 days. Changes of ethology including glucose-consumption during 1 h, crossing and rearing scores of open-field test during 3 min (for assessing the rats' locomoto)and laser Doppler flowmetry values of cortical regional cerebral bloodflow (r CBF) were detected, and Morris water maze test (for assessing the rats' l