p38 mitogen-activated protein kinase inhibitor suppresses the expression of pro-inflammatory cytokines in liver from brain dead rats

• Published in: Zhonghua gan zang bing za zhi Vol. 18; no. 9; p. 703
• Main Authors: Chen, Jie, Liu, Jia-wen, Zeng, Hui-lan, Zeng, Yao-ying, Su, Ze-xuan
• Format: Journal Article
• Language: Chinese
• Published: China 01.09.2010
• Subjects: Animals; Brain Death - metabolism; Imidazoles - pharmacology; Inflammation; Interleukin-1beta - metabolism; Liver - drug effects; Liver - metabolism; Liver - pathology; Male; p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors; p38 Mitogen-Activated Protein Kinases - metabolism; Pyridines - pharmacology; Rats; Rats, Wistar; Signal Transduction; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 1007-3418
• DOI: 10.3760/cma.j.issn.1007-3418.2010.09.014

To observe the suppressive effect on the expression of pro-inflammatory cytokines in liver from brain dead (BD) rats through inhibition of p38 mitogen-activated protein kinase (MAPK) signaling pathway by SB203580. A total of 30 male Wistar rats weighing from 180 to 200 g were randomly divided into 3 experimental groups: (1) BD group (n = 10): brain death was induced in rats; (2) BD+SB203580 group (n = 10): brain death was successfully induced and SB203580 (10 mg/kg) was given through dorsal vein of penis. After brain death artificial ventilation was maintained for 6 hours and only those with mean arterial blood pressure more than 80 mm Hg (1 mm Hg = 0.133 kPa) were accepted as BD donors. (3) Control group (n = 10): living healthy rats. The expressions of TNFalpha and IL-1beta mRNA in liver tissues were analyzed by RT-PCR and the protein expressions of TNFalpha, IL-1beta and phosphorylated p38MAPK were detected by Western blot. The phosphorylated p38MAPK detected in the liver in BD group was significantly incr