Amino-terminal pro-brain natriuretic peptide and brain natriuretic peptide measurements under various detection conditions in patients with chronic heart failure

• Published in: Zhōnghuá xīnxuèguănbìng zázhì Vol. 39; no. 8; p. 695
• Main Authors: Wang, Wei-jia, Zhang, Xiu-ming, Wang, Qian, Wen, Dong-mei, Ouyang, Neng-liang, Cui, Ya-li, Li, Fei
• Format: Journal Article
• Language: Chinese
• Published: China 01.08.2011
• Subjects: Electrochemical Techniques - methods; Heart Failure - blood; Heart Failure - diagnosis; Humans; Immunoassay - methods; Luminescent Measurements - methods; Natriuretic Peptide, Brain - blood; Peptide Fragments - blood; Sensitivity and Specificity; Specimen Handling - methods; Specimen Handling - standards
• ISSN: 0253-3758

To find the potential interference factors for the detection of NT-proBNP and BNP in patients with chronic heart failure. EP15-A2 issued by Clinical and Laboratory Standards Institute (CLSI) was employed to compare the precision and accuracy of commercial NT-proBNP and BNP analyzer electrochemiluminescence immunoassay system Cobas E601 and chemiluminescence system ADVIA Centaur. Moreover, NT-proBNP and BNP were detected in different time interval and in different interfered sampling conditions (haematolysis, choloplania, lipemia). NT-proBNP and BNP of 203 patients with heart failure or heart failure complicated with acute cerebral infarction were analyzed to find the deviation caused by patients' endogenous factors. The precision and accuracy were comparable for NT-proBNP and BNP detection using Cobas E601 and ADVIA Centaur (total-CV below 2.9% and 3.5%, the deviation from definite value below 2.38% and 3.91%). The most suitable sample type for NT-proBNP and BNP detection was serum and EDTA-anticoagulant plas