Inhibitory effects of small interfering RNA targeting c-myc in combination with 5-fluorouracil on the growth in vitro and in vivo

• Published in: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery Vol. 46; no. 7; p. 566
• Main Authors: Sang, Jian-zhong, Wang, Hui-ting, Lou, Wei-hua, Tian, Fang, Jin, Hong-jun, Yuan, Lin-lin
• Format: Journal Article
• Language: Chinese
• Published: China 01.07.2011
• Subjects: Animals; Apoptosis; Cell Line, Tumor; Cell Proliferation - drug effects; Female; Fluorouracil - pharmacology; Gene Silencing; Humans; Laryngeal Neoplasms - pathology; Mice; Mice, Inbred BALB C; Mice, Nude; Proto-Oncogene Proteins c-myc - genetics; RNA, Small Interfering - genetics; Transfection
• ISSN: 1673-0860

To observe the effects of small interfere RNA (siRNA) targeting the c-myc in combination with 5-fluorouracil (5-Fu) on the growth of Hep-2 cells in vitro and in vivo. Hep-2 cells transfected with or without c-myc siRNA were treated with 5-Fu for 48 h. C-myc protein levels in Hep-2 cells were detected using the Western blot. The cell cycle was analyzed by flow cytometry. Hep-2 cells were subcutaneously inoculated into the back of BALB/c nude mice to establish the implanted laryngeal squamous carcinoma model. PBS, c-myc siRNA, and 5-Fu, alone or in combinations were administered i.p. The mice were sacrificed after the treatments and the tumor masses were removed to determine the tumor volume and weight. The inhibitory rate was calculated. Expression of c-myc in tumor tissue was detected by immunocytochemistry and cell apoptosis was analyzed by terminal transferase dUTP nick end labeling (TUNEL). The protein levels of c-myc decreased after transfected with c-myc siRNA. C-myc siRNA-transfected cells showed an inc