Detection of hearing threshold and polymorphic molecular marker analysis of guinea pigs of mimetic aging

• Published in: Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery Vol. 43; no. 11; p. 830
• Main Authors: Mao, Min, Zhang, Jian-guo, Tao, Ai-lin, Yan, Xiao-ling, Ouyang, Shun-lin, Yuan, Xu-ping, Qiu, Li-zi, Xie, Yang, Zou, Ze-hong, Lu, Jie-zhen, Li, Qing
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2008
• Subjects: Aging; Amplified Fragment Length Polymorphism Analysis; Animals; Auditory Threshold; Disease Models, Animal; Evoked Potentials, Auditory, Brain Stem; Female; Galactose - pharmacology; Guinea Pigs; Male; Presbycusis - chemically induced; Presbycusis - physiopathology
• ISSN: 1673-0860

To explore the establishment of the mimetic aging effect in guinea pigs induced by D-galactose, and to detect the biological indicatrix associated with hearing loss and provide a new tool for molecular pathogenesis of hearing loss. Total of 51 guinea pigs were randomly divided into three groups: group A (model aging group, n = 25), which were injected with D-galactose (200 mgxkg(-1)xd(-1)) by intra peritoneum for 6 weeks, group B (model control group, n = 18), which were given the same amount of saline only, and group C (vacant group, n = 15) were not treated. Then, The guinea pigs in group A and B were exposed in noise for 8 days, 8 hours once a day. Auditory brainstem response (ABR) was used to test the hearing threshold of guinea pigs thrice, first before the drug administered, then after 6 weeks the drug used, third after noise exposure. And colorimetry was used to analyze the activity of superoxide dismutase (SOD) and malon dialdehyde (MDA) in brain and liver tissue. The DNA of inner ear tissue was harve