Effects of lycopene on cerebral ischemia-reperfusion injury in rats

• Published in: Wei sheng yan jiu Vol. 39; no. 2; p. 201
• Main Authors: Wei, Yan, Shen, Xinnan, Shen, Hui, Mai, Jiayi, Wu, Min, Yao, Guoying
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2010
• Subjects: Animals; Antioxidants - pharmacology; Brain Ischemia - pathology; Carotenoids - pharmacology; Hypoxia-Inducible Factor 1, alpha Subunit - genetics; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Male; Neuroprotective Agents - pharmacology; Oxidative Stress - drug effects; Rats; Rats, Sprague-Dawley; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; RNA, Messenger - genetics; RNA, Messenger - metabolism
• ISSN: 1000-8020

To study the protective effects of lycopene (LP) on cerebral ischemia-reperfusion injury and oxidative stress in SD rats and the mechanism of them. The rats were divided into five groups: normal control group, model control group, sham group and two LP groups (fed with 5 mg/kg bw or 20 mg/kg bw of lycopene daily for 15 days). The model for cerebral ischemia-reperfusion injury was established by middle cerebral artery occlusion (MCAO). The score of neurological behavior was evaluated at the 3rd and 24th hours after reperfusion. The rats were put to death 24 h after reperfusion. The size of cerebral infarction was measured. The activities of iNOS, SOD, CAT and the contents of NO and MDA in brain and serum uric acid were measured. The expressions of Bcl-2 mRNA and HIF-1alphamRNA in cortex were examined by using reverse transcription polymerase chain reaction (RT-PCR) technique. In comparison with the model group, the neurological deficits were milder, the volumes of cerebral infarction were smaller, the activiti