Protection mechanisms of ATP-sensitive K channels on hippocampal CA1 neurons during chronic severe hypoxia

To study the protection mechanisms of K(ATP) channels on hippocampal CA1 neurons during chronic severe hypoxia. p53 expression, DNA fraction, and cell apoptosis were examined in cultured hippocampal neurons in control group, hypoxia group, hypoxia group treated with K(ATP) channels antagonist and hy...

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Published inZhongguo ying yong sheng li xue za zhi Vol. 23; no. 3; p. 257
Main Authors Huang, Lian-Yan, Li, Wen-Jun, Li, Bo-Xing, Zou, Fei
Format Journal Article
LanguageChinese
Published China 01.08.2007
Subjects
Adenosine Triphosphate - metabolism
Animals
Apoptosis
Cell Hypoxia
Cell Survival
Diazoxide - pharmacology
Genes, p53
Hippocampus - cytology
Hippocampus - metabolism
KATP Channels - antagonists & inhibitors
KATP Channels - metabolism
Neurons - cytology
Neurons - metabolism
Patch-Clamp Techniques
Rats
Rats, Sprague-Dawley
Tolbutamide - pharmacology
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Summary:To study the protection mechanisms of K(ATP) channels on hippocampal CA1 neurons during chronic severe hypoxia. p53 expression, DNA fraction, and cell apoptosis were examined in cultured hippocampal neurons in control group, hypoxia group, hypoxia group treated with K(ATP) channels antagonist and hypoxia group treated with K(ATP) channels agonist. In the group of a 12 h long exposure to oxygen concentration of 0%, diazoxide (100 micromol/L), the K(ATP) channels agonist, reduced p53 expression and the hypoxia-induced apoptosis. In contrast, tolbutamide (100 micromol/L), the K(ATP) channels antagonist, significantly rose p53 expression and the hypoxia-induced apoptosis, which could be reversed by p53 inhibitor TSA. K(ATP) channels protect hippocampal neurons against chronic severe hypoxia by suppressing p53 expression.
ISSN:1000-6834