FMS-like tyrosine kinase 3 gene mutation in acute myeloid leukemia detected by denaturing PAGE and its clinical significance

The aim of this study was to analyze the frequency of flt3 length mutation (flt3-LM) in de novo acute myeloid leukemia patients and the relationship between flt3-LM and chromosome alterations, FAB subgroups, as well as efficiency of therapy. Genomic DNA was amplified by PCR; 2% agarose gel or 8% den...

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Published inZhongguo shi yan xue ye xue za zhi Vol. 18; no. 6; p. 1386
Main Authors Ma, Liang, Zhong, Ming-Hua, Feng, Dai-Rong, Long, Hong, Shen, Jun, Ma, Yi-Gai, Huang, Shang-Zhi
Format Journal Article
LanguageChinese
Published China 01.12.2010
Subjects
Adolescent
Adult
Aged
Aged, 80 and over
Case-Control Studies
Female
fms-Like Tyrosine Kinase 3 - genetics
Humans
Karyotyping
Leukemia, Myeloid, Acute - genetics
Male
Middle Aged
Mutation
Young Adult
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Summary:The aim of this study was to analyze the frequency of flt3 length mutation (flt3-LM) in de novo acute myeloid leukemia patients and the relationship between flt3-LM and chromosome alterations, FAB subgroups, as well as efficiency of therapy. Genomic DNA was amplified by PCR; 2% agarose gel or 8% denaturing PAGE were used to detect the length mutation of flt3 gene in 99 de novo acute myeloid leukemia patients; karyotyping in 72 AML patients was performed by G banding technique. The results showed that the flt3-LM was detected in 20.2% (20/99) patients by agarose gel electrophoresis, and in 29.9% (29/99) by denaturing PAGE. The flt3-LM was not detected in M(0) (only one patient was available), but flt3-LM occurrence in AML subtypes was as follow: in M(2) (9/30), M(3) (6/27), M(4) (4/14), M(5) (7/19), M(6) (3/8) respectively. flt3-LM in patients with normal karyotypes (39.13%) was more prevalent as compared with patients of abnormal karyotype (24.49%), but there was no statistical difference (p > 0.05). The comp
ISSN:1009-2137