Expression of CREB/Bcl-2 in bone marrow mononuclear cells of children with acute leukemia

• Published in: Zhongguo dang dai er ke za zhi Vol. 12; no. 3; p. 177
• Main Authors: Wen, Chuan, Ma, Fu-Tian, Wan, Wu-Qing
• Format: Journal Article
• Language: Chinese
• Published: China 01.03.2010
• Subjects: Acute Disease; Adolescent; Bone Marrow Cells - metabolism; Child; Child, Preschool; Cyclic AMP Response Element-Binding Protein - analysis; Cyclic AMP Response Element-Binding Protein - genetics; Female; Humans; Infant; Leukemia - metabolism; Male; Proto-Oncogene Proteins c-bcl-2 - analysis; Proto-Oncogene Proteins c-bcl-2 - genetics; RNA, Messenger - analysis
• ISSN: 1008-8830

To study the expression and role of cyclic-AMP response binding protein (CREB) and Bcl-2 in children with acute leukemia. Ninety-two children with acute leukemia (leukemia group) and 30 children with non-hematologic malignancies (control group) were enrolled. The mRNA and protein expression of CREB and Bcl-2 in bone marrow mononuclear cells were measured by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot. The mRNA and protein expression of CREB and Bcl-2 in the leukemia group was significantly higher than that in the control group (p<0.01). There were no significant differences in the expression of CREB and Bcl-2 between acute lymphoblastic leukemia and acute myeloid leukemia subgroups. At the initial diagnosis, the mRNA and protein expression of CREB and Bcl-2 in children with extramedullary infiltration was higher than that in children without (p<0.05). In the leukemia group, the mRNA and protein expression of CREB and Bcl-2 in the complete remission subgroup was significantly lowe