Association of 1233A/G polymorphism of angiopoietin-2 gene with type 2 diabetes mellitus and diabetic nephropathy

• Published in: Zhonghua yi xue yi chuan xue za zhi Vol. 29; no. 1; p. 72
• Main Authors: He, Quan, Luo, Hai-ming, Zhu, Bao-sheng, Tang, Xin-hua, Jiang, Lu-zhi
• Format: Journal Article
• Language: Chinese
• Published: China 01.02.2012
• Subjects: Angiopoietin-2 - genetics; Case-Control Studies; Chemokine CCL2 - genetics; Diabetes Mellitus, Type 2 - genetics; Diabetic Nephropathies - genetics; Gene Frequency; Genotype; Humans; Polymorphism, Single Nucleotide
• ISSN: 1003-9406
• DOI: 10.3760/cma.j.issn.1003-9406.2012.01.018

To investigate the association between single nucleotide polymorphisms (SNP) of angiopoietin-2 (Ang-2) gene and type 2 diabetes mellitus (T2DM) and diabetic nephropathy (DN). Genotype and allele frequency of Ang-2 were detected by amplification refractory mutation system-PCR(ARMS-PCR) in 221 cases with T2DM and 104 normal controls. Monocyte chemoattractant protein-1(MCP-1) was measured by ELISA. DN patients were divided into three groups according to urinary albumin excretion rates (UAER), i.e. DN0: UAER < 30 mg/24 h, DN1: UAER 30-300 mg/24 h and DN2: UAER > 300 mg/24 h. Statistics showed: (1) Genotype frequencies and allele frequencies in Ang-2 1233A/G had significant difference but not Ang-2 759T/G and 1078A/G; (2) Comparing with those with genotype AA, the relative risk of genotype (AG+ GG) suffered from T2DM and DN were 2.265 fold (OR= 2.265, 95% CI: 1.223-1.402, P= 0.031), 1.789 fold (OR= 1.789, 95% CI: 0.889-1.021, P= 0.012), respectively; (3) The onset of DN was related to Ang-2 1233A/G allele G (r= 1.