Induction of anti-lymphoma cytotoxic T cell effect by dendritic cells transfected with recombinant adenovirus vectors carrying survivin gene

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 15; no. 3; p. 591
• Main Authors: Zhu, Xiong-Peng, Chen, Zhi-Zhe, Hu, Jian-Da, Li, Chun-Tuan, Yang, Ting, Xu, Zheng-Shu
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2007
• Subjects: Adenoviridae - genetics; Adenoviridae - metabolism; Apoptosis - immunology; Dendritic Cells - immunology; Genetic Vectors - genetics; Humans; Inhibitor of Apoptosis Proteins; Interleukin-12 - metabolism; Lymphoma - immunology; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - immunology; Recombination, Genetic; T-Lymphocytes, Cytotoxic - immunology; Transfection; Tumor Cells, Cultured
• ISSN: 1009-2137

This study was purposed to investigate the immunological effects of modified dendritic cells (DCs) in inducing cytotoxic T cells (CTLs) effect against lymphoma cells. The DCs were derived from human peripheral blood and transfected with recombined adenovirus vector carrying survivin gene, Western blot was used to detect the expression of survivin, the lactate dehydrogenase (LDH) release test was used to determine the cytotoxicity of CTLs, the mixed lymphocyte reaction (MLR) was used to measure the ability to proleferate allo-lymphocyte by DCs, ELISA was used to assay IL-12 level in supernatant. The results showed that the expression of survivin in transfected dentritic cells was confirmed by Western blot analysis. In MLR assay, DCs transfected with Ad-survivin could induce higher allogeneic lymphocytes reaction at the ratios of 1:5, 1:10, 1:50 and 1:100. DCs transfected with Ad-survivin had much higher activity of CTL to CA46 cells than control DCs. The levels of IL-12 of supernatants containing DCs transfect